Composite

Part:BBa_K4616888:Design

Designed by: Haoyu Zhang   Group: iGEM23_BIT-China   (2023-10-04)


Optimized production lines of Sclareol in S.cerevisiae


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 67
    Illegal EcoRI site found at 448
    Illegal EcoRI site found at 1648
    Illegal EcoRI site found at 3001
    Illegal EcoRI site found at 3547
    Illegal XbaI site found at 19
    Illegal XbaI site found at 973
    Illegal XbaI site found at 1015
    Illegal XbaI site found at 2428
    Illegal XbaI site found at 2482
    Illegal XbaI site found at 3670
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 67
    Illegal EcoRI site found at 448
    Illegal EcoRI site found at 1648
    Illegal EcoRI site found at 3001
    Illegal EcoRI site found at 3547
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 67
    Illegal EcoRI site found at 448
    Illegal EcoRI site found at 1648
    Illegal EcoRI site found at 3001
    Illegal EcoRI site found at 3547
    Illegal BglII site found at 1678
    Illegal BglII site found at 2102
    Illegal BglII site found at 2138
    Illegal BglII site found at 2479
    Illegal BglII site found at 2587
    Illegal BglII site found at 2905
    Illegal BglII site found at 2918
    Illegal BamHI site found at 1607
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 67
    Illegal EcoRI site found at 448
    Illegal EcoRI site found at 1648
    Illegal EcoRI site found at 3001
    Illegal EcoRI site found at 3547
    Illegal XbaI site found at 19
    Illegal XbaI site found at 973
    Illegal XbaI site found at 1015
    Illegal XbaI site found at 2428
    Illegal XbaI site found at 2482
    Illegal XbaI site found at 3670
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 67
    Illegal EcoRI site found at 448
    Illegal EcoRI site found at 1648
    Illegal EcoRI site found at 3001
    Illegal EcoRI site found at 3547
    Illegal XbaI site found at 19
    Illegal XbaI site found at 973
    Illegal XbaI site found at 1015
    Illegal XbaI site found at 2428
    Illegal XbaI site found at 2482
    Illegal XbaI site found at 3670
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1952


Design Notes

NO


Source

NO

References